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notch4  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology notch4
    Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or <t>Notch4</t> in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )
    Notch4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling"

    Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling

    Journal: Signal Transduction and Targeted Therapy

    doi: 10.1038/s41392-025-02432-3

    Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )
    Figure Legend Snippet: Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

    Techniques Used: Western Blot, Expressing, RNA Sequencing, Derivative Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Inactivation of Notch4 Attenuated Pancreatic Tumorigenesis in Mice
    Article Snippet: .. The primary antibodies used for IHC were Notch4 (1:1,000, Notch4-ICD, intracellular domain of Notch4), kindly provided by Dr. Carrie J. Shawber; refs. ), Amylase (1:100, catalog no.: sc-46657, Santa Cruz Biotechnology), CK19 (1:500, catalog no.: ab52625, Abcam), Pcsk5 (1:5,000, catalog no.: PA5-42378, Thermo Fisher Scientific), and endomucin (1:1,000, sc-65495, Santa Cruz Biotechnology). .. The primary antibodies used for IF were Notch4 (1:100, Notch4-ICD, kindly provided by Dr. Carrie J. Shawber; ref. ), Amylase (1:100, catalog no.: sc-46657, Santa Cruz Biotechnology), CK19 (1:25, TROMA3; DSHB).

    Incubation:

    Article Title: Preliminary Research of Main Components of Dll4/ Notch-VEGF Signaling Pathway Under High-Glucose Stimulation in vitro
    Article Snippet: .. After the proteins were transferred on nitrocellulose membranes, antibodies specific for Dll4 (1:200, SC365429, Santa Cruz, CA), Notch1 (1:200, SC376403, Santa Cruz, CA), Notch4 (1:200, SC393893, Santa Cruz, CA) and the VEGF (1:200, SC365578, Santa Cruz, CA) were used for incubation. .. Band intensity was analyzed with a gel imaging analysis system (Image Lab, Bio-Rad, USA).

    Article Title: Lumiflavin Reduces Cisplatin Resistance in Cancer Stem-Like Cells of OVCAR-3 Cell Line by Inducing Differentiation
    Article Snippet: .. The proteins were then transferred to PVDF membranes and incubated at 4°C overnight with primary antibodies Jagged1 (Jag1,Abcam,1:1000), Jagged2 (Jag2, Abcam,1:1000), Delta-like canonical notch ligand 1 (Dll1, Abcam,1:500), Delta-like canonical notch ligand 3 (Dll3, Cell Signaling Technology,1:1000), Delta-like canonical notch ligand 4 (Dll4, Cell Signaling Technology,1:1000), Notch1 (Abcam,1:1000), Notch2 (Abcam,1:5000), Notch3(Santa Cruz, 1:500), Notch4 (Santa Cruz, 1:500) or NICD (Cell Signaling Technology, 1:1000). .. Membranes were washed and incubated with anti-rabbit/anti-mouse antibodies (MultiSciences Biotech, 1:5000) for 1 h at room temperature.

    Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling.
    Article Snippet: Protein samples were then separated on 10% Tris‒glycine gels (BIOMIKY, Cat# MK303) and transferred to nitrocellulose membranes (Merck Millipore, Cat# ISEQ00010). .. The membranes were blocked with 5% nonfat milk in Signal Transduction and Targeted Therapy (2025) 10:334 TBST (0.1% Tween-20 in TBS) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against N1ICD (1:1000, CST, Cat# 8216S), N2ICD (1:1000, CST, Cat# 4970T), β-actin (1:1000, CST, Cat# 5732T), Notch3 (1:1000, Santa Cruz, Cat# sc515825), or Notch4 (1:1000, Santa Cruz, Cat# sc-393893). .. After three washes with TBST, the membranes were incubated with HRP-conjugated secondary antibodies—goat anti-rabbit IgG (1:1000, Beyotime, Cat# A0208) or goat anti-mouse IgG (1:1000, Beyotime, Cat# A0216)—for 2 h at room temperature.

    Article Title: MAML3 Contributes to Induction of Malignant Phenotype of Gallbladder Cancer Through Morphogenesis Signalling Under Hypoxia.
    Article Snippet: .. The protein-transferred membranes (Whatman GmbH, Dassel, Germany) were incubated with primary antibodies against MAML3 (1:5000; Mouse. no. A300-684A; ThermoFisher Scientific, Waltham, MA, USA), Ki 67 (1:200; cat. no. sc 15402; Santa Cruz Biotechnology, Dallas, TX, USA), E-cadherin (1:200; cat. no. sc 7870; Santa Cruz Biotechnology), vimentin (1:1,000; cat. no. ab92547; Abcam, Cambridge, UK), SNAI-1 (1:200, cat. no. sc 271977; Santa Cruz Biotechnology), Slug (1:200; cat. no. sc 15391; Santa Cruz Biotechnology), α-SMA (1:2000; Rabbit; ab5694; Abcam), PTCH1 (1:200; Santa Cruz Biotechnology, Inc.), SMO (1:200; cat. no. 20787 1 AP; ProteinTech Group, Rosemont, IL, USA), GLI1 (1:500; cat. no. ab151796; Abcam), GLI2 (1:500; cat. no. ab187386; Abcam), GLI3 (1:200; cat.no.sc 6154; Santa Cruz Biotechnology), HES1 (1:100; goat, sc-13844; Santa Cruz Biotechnology), NOTCH1 (1:200; Rabbit; c-20, sc-6014-R; Santa Cruz Biotechnology), NOTCH2 (1:200; Rabbit; 25-255, sc-5545; Santa Cruz Biotechnology), NOTCH3 (1:200; Rabbit; M-134, sc-5593; Santa Cruz Biotechnology), and NOTCH4 (1:200; Rabbit; H-225, sc5594; Santa Cruz Biotechnology) overnight at 4 ̊C. .. The membranes were incubated for 1 h at room temperature (25-28 ̊C) with horseradish anti-mouse (1:10,000; cat. no. NA931; Merck, Cytiva, Marlborough, MA, USA) peroxidase-conjugated antibody, antirabbit (1:10,000; cat. no. NA934; Merck, Cytiva) horseradish peroxidase-linked antibody, or anti-goat (1:10,000; cat. no. sc 2020; Santa Cruz Biotechnology, Dallas, TX, USA) horseradish peroxidase-linked antibody. α-Tubulin (1:1,000; cat. no. T6199; Sigma Aldrich; Merck KGaA, Darmstadt, Germany) was used as the protein loading control.

    Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling
    Article Snippet: Protein samples were then separated on 10% Tris‒glycine gels (BIOMIKY, Cat# MK303) and transferred to nitrocellulose membranes (Merck Millipore, Cat# ISEQ00010). .. The membranes were blocked with 5% nonfat milk in TBST (0.1% Tween-20 in TBS) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against N1ICD (1:1000, CST, Cat# 8216S), N2ICD (1:1000, CST, Cat# 4970T), β-actin (1:1000, CST, Cat# 5732T), Notch3 (1:1000, Santa Cruz, Cat# sc-515825), or Notch4 (1:1000, Santa Cruz, Cat# sc-393893). .. After three washes with TBST, the membranes were incubated with HRP-conjugated secondary antibodies—goat anti-rabbit IgG (1:1000, Beyotime, Cat# A0208) or goat anti-mouse IgG (1:1000, Beyotime, Cat# A0216)—for 2 h at room temperature.

    Transduction:

    Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling.
    Article Snippet: Protein samples were then separated on 10% Tris‒glycine gels (BIOMIKY, Cat# MK303) and transferred to nitrocellulose membranes (Merck Millipore, Cat# ISEQ00010). .. The membranes were blocked with 5% nonfat milk in Signal Transduction and Targeted Therapy (2025) 10:334 TBST (0.1% Tween-20 in TBS) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against N1ICD (1:1000, CST, Cat# 8216S), N2ICD (1:1000, CST, Cat# 4970T), β-actin (1:1000, CST, Cat# 5732T), Notch3 (1:1000, Santa Cruz, Cat# sc515825), or Notch4 (1:1000, Santa Cruz, Cat# sc-393893). .. After three washes with TBST, the membranes were incubated with HRP-conjugated secondary antibodies—goat anti-rabbit IgG (1:1000, Beyotime, Cat# A0208) or goat anti-mouse IgG (1:1000, Beyotime, Cat# A0216)—for 2 h at room temperature.



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    Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or <t>Notch4</t> in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )
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    Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or <t>Notch4</t> in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )
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    Image Search Results


    Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling

    doi: 10.1038/s41392-025-02432-3

    Figure Lengend Snippet: Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

    Article Snippet: The membranes were blocked with 5% nonfat milk in TBST (0.1% Tween-20 in TBS) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against N1ICD (1:1000, CST, Cat# 8216S), N2ICD (1:1000, CST, Cat# 4970T), β-actin (1:1000, CST, Cat# 5732T), Notch3 (1:1000, Santa Cruz, Cat# sc-515825), or Notch4 (1:1000, Santa Cruz, Cat# sc-393893).

    Techniques: Western Blot, Expressing, RNA Sequencing, Derivative Assay